cdna synthesis kit Search Results


97
Genecopoeia first strand cdna synthesis kit
First Strand Cdna Synthesis Kit, supplied by Genecopoeia, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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TaKaRa smart race cdna amplification kit user manual
Smart Race Cdna Amplification Kit User Manual, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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New England Biolabs protoscript ii first strand cdna synthesis kit
Protoscript Ii First Strand Cdna Synthesis Kit, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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New England Biolabs first strand cdna synthesis
First Strand Cdna Synthesis, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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96
Solis BioDyne rt cdna synthesis kit
BAPTA-AM induced sensitization by sub-toxic-dose of cisplatin is associated with a decrease in mRNA expression of p21, calmodulin, S100A8, and S100A14. A. Cells were exposed to sub-toxic (20 μM), 40 μM, and 100 μM of cisplatin for 18 h, in the presence or absence of BAPTA-AM. After treatment, total RNA was extracted from cells followed by <t>cDNA</t> synthesis and real-time qPCR. All mRNA expression levels were normalized against GAPDH. The data show the normalized expression of (A) p21, (B) calmodulin, (C) S100A8, and (D) S100A14, relative to the expression of the control. Each value is the mean ± SEM. Experiments were conducted with triplicate samples and repeated at least twice. Statistically significant differences were determined by one-way ANOVA. Statistical comparison between two groups was performed using t -test, ∗ P <0.05, ∗∗ P <0.01, ∗∗∗ P <0.001.
Rt Cdna Synthesis Kit, supplied by Solis BioDyne, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rt cdna synthesis kit - by Bioz Stars, 2026-02
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97
Genecopoeia total rna
BAPTA-AM induced sensitization by sub-toxic-dose of cisplatin is associated with a decrease in mRNA expression of p21, calmodulin, S100A8, and S100A14. A. Cells were exposed to sub-toxic (20 μM), 40 μM, and 100 μM of cisplatin for 18 h, in the presence or absence of BAPTA-AM. After treatment, total RNA was extracted from cells followed by <t>cDNA</t> synthesis and real-time qPCR. All mRNA expression levels were normalized against GAPDH. The data show the normalized expression of (A) p21, (B) calmodulin, (C) S100A8, and (D) S100A14, relative to the expression of the control. Each value is the mean ± SEM. Experiments were conducted with triplicate samples and repeated at least twice. Statistically significant differences were determined by one-way ANOVA. Statistical comparison between two groups was performed using t -test, ∗ P <0.05, ∗∗ P <0.01, ∗∗∗ P <0.001.
Total Rna, supplied by Genecopoeia, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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94
Miltenyi Biotec cdna synthesis kit
BAPTA-AM induced sensitization by sub-toxic-dose of cisplatin is associated with a decrease in mRNA expression of p21, calmodulin, S100A8, and S100A14. A. Cells were exposed to sub-toxic (20 μM), 40 μM, and 100 μM of cisplatin for 18 h, in the presence or absence of BAPTA-AM. After treatment, total RNA was extracted from cells followed by <t>cDNA</t> synthesis and real-time qPCR. All mRNA expression levels were normalized against GAPDH. The data show the normalized expression of (A) p21, (B) calmodulin, (C) S100A8, and (D) S100A14, relative to the expression of the control. Each value is the mean ± SEM. Experiments were conducted with triplicate samples and repeated at least twice. Statistically significant differences were determined by one-way ANOVA. Statistical comparison between two groups was performed using t -test, ∗ P <0.05, ∗∗ P <0.01, ∗∗∗ P <0.001.
Cdna Synthesis Kit, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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99
TaKaRa primescripttm 1 st strand cdna synthesis kit
BAPTA-AM induced sensitization by sub-toxic-dose of cisplatin is associated with a decrease in mRNA expression of p21, calmodulin, S100A8, and S100A14. A. Cells were exposed to sub-toxic (20 μM), 40 μM, and 100 μM of cisplatin for 18 h, in the presence or absence of BAPTA-AM. After treatment, total RNA was extracted from cells followed by <t>cDNA</t> synthesis and real-time qPCR. All mRNA expression levels were normalized against GAPDH. The data show the normalized expression of (A) p21, (B) calmodulin, (C) S100A8, and (D) S100A14, relative to the expression of the control. Each value is the mean ± SEM. Experiments were conducted with triplicate samples and repeated at least twice. Statistically significant differences were determined by one-way ANOVA. Statistical comparison between two groups was performed using t -test, ∗ P <0.05, ∗∗ P <0.01, ∗∗∗ P <0.001.
Primescripttm 1 St Strand Cdna Synthesis Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Bio-Rad iscript select cdna synthesis kit
BAPTA-AM induced sensitization by sub-toxic-dose of cisplatin is associated with a decrease in mRNA expression of p21, calmodulin, S100A8, and S100A14. A. Cells were exposed to sub-toxic (20 μM), 40 μM, and 100 μM of cisplatin for 18 h, in the presence or absence of BAPTA-AM. After treatment, total RNA was extracted from cells followed by <t>cDNA</t> synthesis and real-time qPCR. All mRNA expression levels were normalized against GAPDH. The data show the normalized expression of (A) p21, (B) calmodulin, (C) S100A8, and (D) S100A14, relative to the expression of the control. Each value is the mean ± SEM. Experiments were conducted with triplicate samples and repeated at least twice. Statistically significant differences were determined by one-way ANOVA. Statistical comparison between two groups was performed using t -test, ∗ P <0.05, ∗∗ P <0.01, ∗∗∗ P <0.001.
Iscript Select Cdna Synthesis Kit, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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97
Quanta Biosciences qscript cdna synthesis kit
BAPTA-AM induced sensitization by sub-toxic-dose of cisplatin is associated with a decrease in mRNA expression of p21, calmodulin, S100A8, and S100A14. A. Cells were exposed to sub-toxic (20 μM), 40 μM, and 100 μM of cisplatin for 18 h, in the presence or absence of BAPTA-AM. After treatment, total RNA was extracted from cells followed by <t>cDNA</t> synthesis and real-time qPCR. All mRNA expression levels were normalized against GAPDH. The data show the normalized expression of (A) p21, (B) calmodulin, (C) S100A8, and (D) S100A14, relative to the expression of the control. Each value is the mean ± SEM. Experiments were conducted with triplicate samples and repeated at least twice. Statistically significant differences were determined by one-way ANOVA. Statistical comparison between two groups was performed using t -test, ∗ P <0.05, ∗∗ P <0.01, ∗∗∗ P <0.001.
Qscript Cdna Synthesis Kit, supplied by Quanta Biosciences, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Vazyme Biotech Co hiscript iii 1st strand cdna synthesis kit
BAPTA-AM induced sensitization by sub-toxic-dose of cisplatin is associated with a decrease in mRNA expression of p21, calmodulin, S100A8, and S100A14. A. Cells were exposed to sub-toxic (20 μM), 40 μM, and 100 μM of cisplatin for 18 h, in the presence or absence of BAPTA-AM. After treatment, total RNA was extracted from cells followed by <t>cDNA</t> synthesis and real-time qPCR. All mRNA expression levels were normalized against GAPDH. The data show the normalized expression of (A) p21, (B) calmodulin, (C) S100A8, and (D) S100A14, relative to the expression of the control. Each value is the mean ± SEM. Experiments were conducted with triplicate samples and repeated at least twice. Statistically significant differences were determined by one-way ANOVA. Statistical comparison between two groups was performed using t -test, ∗ P <0.05, ∗∗ P <0.01, ∗∗∗ P <0.001.
Hiscript Iii 1st Strand Cdna Synthesis Kit, supplied by Vazyme Biotech Co, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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BAPTA-AM induced sensitization by sub-toxic-dose of cisplatin is associated with a decrease in mRNA expression of p21, calmodulin, S100A8, and S100A14. A. Cells were exposed to sub-toxic (20 μM), 40 μM, and 100 μM of cisplatin for 18 h, in the presence or absence of BAPTA-AM. After treatment, total RNA was extracted from cells followed by cDNA synthesis and real-time qPCR. All mRNA expression levels were normalized against GAPDH. The data show the normalized expression of (A) p21, (B) calmodulin, (C) S100A8, and (D) S100A14, relative to the expression of the control. Each value is the mean ± SEM. Experiments were conducted with triplicate samples and repeated at least twice. Statistically significant differences were determined by one-way ANOVA. Statistical comparison between two groups was performed using t -test, ∗ P <0.05, ∗∗ P <0.01, ∗∗∗ P <0.001.

Journal: Heliyon

Article Title: Modulation of calcium-binding proteins expression and cisplatin chemosensitivity by calcium chelation in human breast cancer MCF-7 cells

doi: 10.1016/j.heliyon.2021.e06041

Figure Lengend Snippet: BAPTA-AM induced sensitization by sub-toxic-dose of cisplatin is associated with a decrease in mRNA expression of p21, calmodulin, S100A8, and S100A14. A. Cells were exposed to sub-toxic (20 μM), 40 μM, and 100 μM of cisplatin for 18 h, in the presence or absence of BAPTA-AM. After treatment, total RNA was extracted from cells followed by cDNA synthesis and real-time qPCR. All mRNA expression levels were normalized against GAPDH. The data show the normalized expression of (A) p21, (B) calmodulin, (C) S100A8, and (D) S100A14, relative to the expression of the control. Each value is the mean ± SEM. Experiments were conducted with triplicate samples and repeated at least twice. Statistically significant differences were determined by one-way ANOVA. Statistical comparison between two groups was performed using t -test, ∗ P <0.05, ∗∗ P <0.01, ∗∗∗ P <0.001.

Article Snippet: Total RNA was extracted from MCF-7 cells using the RNeasy Mini kit (Qiagen, Valencia, CA, USA). cDNA was produced from 1.8 μg of total RNA by FIREScript RT cDNA Synthesis KIT (Solis BioDyne, Tartu, Estonia) using random primers following manufacturer's protocol.

Techniques: Expressing, cDNA Synthesis, Control, Comparison