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Image Search Results
Journal: Heliyon
Article Title: Modulation of calcium-binding proteins expression and cisplatin chemosensitivity by calcium chelation in human breast cancer MCF-7 cells
doi: 10.1016/j.heliyon.2021.e06041
Figure Lengend Snippet: BAPTA-AM induced sensitization by sub-toxic-dose of cisplatin is associated with a decrease in mRNA expression of p21, calmodulin, S100A8, and S100A14. A. Cells were exposed to sub-toxic (20 μM), 40 μM, and 100 μM of cisplatin for 18 h, in the presence or absence of BAPTA-AM. After treatment, total RNA was extracted from cells followed by cDNA synthesis and real-time qPCR. All mRNA expression levels were normalized against GAPDH. The data show the normalized expression of (A) p21, (B) calmodulin, (C) S100A8, and (D) S100A14, relative to the expression of the control. Each value is the mean ± SEM. Experiments were conducted with triplicate samples and repeated at least twice. Statistically significant differences were determined by one-way ANOVA. Statistical comparison between two groups was performed using t -test, ∗ P <0.05, ∗∗ P <0.01, ∗∗∗ P <0.001.
Article Snippet: Total RNA was extracted from MCF-7 cells using the RNeasy Mini kit (Qiagen, Valencia, CA, USA). cDNA was produced from 1.8 μg of total RNA by FIREScript
Techniques: Expressing, cDNA Synthesis, Control, Comparison